Prep HPLC Column, HPS Silica, 5um, 120A. 21.2mm ID x 100mm Length. Cogent Brand. 1 EA.
Additional Info:
75001-P100 Cogent HPS Silica™ Prep HPLC Column is packed with high‑purity, unmodified silica, providing consistent and reproducible performance for preparative separations of polar and hydrophilic compounds.
Because the stationary phase is unbonded and unmodified, it delivers strong surface silanol interactions, making it ideal for:
Normal Phase chromatography (primary mode)
HILIC separations for polar analytes (highly suitable secondary mode)
This dual‑mode capability increases method flexibility, allowing laboratories to optimize separations using either non‑polar normal phase solvents or high‑organic HILIC conditions.
This proprietary HPS Silica™ phase has been widely used for many years as a premium-value alternative to columns such as Inertsil® and Luna®, offering dependable selectivity and reproducibility across a wide range of applications.
The 5 µm particle size is well suited for preparative workflows, providing:
Lower backpressure vs smaller particles
Higher allowable flow rates
Robust performance during repeated purification cycles
100 mm Length – Balanced Resolution for Preparative Workflows
The 100 mm length provides an effective balance between resolution and throughput.
Higher resolution than 50 mm preparative columns
Faster cycle times than 150 mm or 250 mm formats
Increased peak capacity for moderately complex mixtures
✅ Recommended for routine preparative purification and process development
21.2 mm ID – Preparative Scale
Designed for preparative HPLC purification
High sample loading capability
Suitable for analytical‑to‑prep scale‑up
Compatible with large‑volume injections and fraction collection systems
✅ Recommended for scale‑up and production‑level purification workflows
Flow Rate & System Requirements (21.2 mm ID, 5 µm)
⚠️ Requires preparative or process‑scale HPLC system capability
Typical flow rate range: ~15 – 40 mL/min
Low‑to‑moderate backpressure
Designed for high‑flow purification
Compatible with preparative detectors and fraction collectors
✅ Important: A specialized preparative HPLC system is required for columns of this size
Why Choose HPS Silica™ for Preparative HPLC
Unmodified Silica – Dual Mode Capability
Ideal for Normal Phase separations
Highly suitable for HILIC separations of polar compounds
High‑Purity Silica Surface
Strong retention of hydrophilic analytes
Consistent, reproducible selectivity
USP L3 Compatibility
Suitable for compendial and validated methods
Reliable alternative to legacy silica phases
Proven Performance
Widely used across analytical and preparative labs
Long-term, dependable results
Performance Advantages
Low Backpressure (5 µm Particles)
Enables higher flow rates for purification
Compatible with standard preparative systems
Fast Equilibration
Reduces cycle time between runs
Flexible Selectivity
Supports both Normal Phase and HILIC method development
Typical Applications
Preparative purification of lipids and fats
Polar compound isolation
Normal phase and HILIC separations
Process scale‑up from analytical HPLC
Pharmaceutical and chemical purification workflows
Important Usage Notes
Suitable for both Normal Phase and HILIC chromatography conditions
Use appropriate non‑polar solvents (NP) or high‑organic mobile phases (HILIC)
Requires preparative HPLC system capability
Ensure proper equilibration for reproducible retention
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable